transam p53 transcription factor elisa kit Search Results


transam p53 elisa kit  (Active Motif)
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Active Motif transam p53 elisa kit
A, Overlapped target genes of NF-κB and <t>p53.</t> B, Gene regulatory programs of seven TFs (NF-κB, p53, AP1, CEBPB, EGR1, SP1, and STAT3). Genes in underlined, bold and bold-underlined refer to known targets of NF-κB, p53, and both NF-κB/p53, respectively. ↓ and ↑ refer to number of predicted target genes differentially over- and under-expressed in the tumor cells, respectively.
Transam P53 Elisa Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A, Overlapped target genes of NF-κB and p53. B, Gene regulatory programs of seven TFs (NF-κB, p53, AP1, CEBPB, EGR1, SP1, and STAT3). Genes in underlined, bold and bold-underlined refer to known targets of NF-κB, p53, and both NF-κB/p53, respectively. ↓ and ↑ refer to number of predicted target genes differentially over- and under-expressed in the tumor cells, respectively.

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: A, Overlapped target genes of NF-κB and p53. B, Gene regulatory programs of seven TFs (NF-κB, p53, AP1, CEBPB, EGR1, SP1, and STAT3). Genes in underlined, bold and bold-underlined refer to known targets of NF-κB, p53, and both NF-κB/p53, respectively. ↓ and ↑ refer to number of predicted target genes differentially over- and under-expressed in the tumor cells, respectively.

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques:

Enriched signal pathways in the gene regulatory programs of NF-κB and p53.

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: Enriched signal pathways in the gene regulatory programs of NF-κB and p53.

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques:

A, network of the wt p53-deficient cells. B, network of the mt p53 cells. Every node represents a common target gene of NF-κB, p53, mir21 or mir34ac, and was annotated according to inflammatory and immune responses (green nodes), apoptosis (blue), angiogenesis (yellow), proliferation (red), adhesion (gold), proteolysis (light red) and other processes (light blue). The networks were presented by cytoscape.

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: A, network of the wt p53-deficient cells. B, network of the mt p53 cells. Every node represents a common target gene of NF-κB, p53, mir21 or mir34ac, and was annotated according to inflammatory and immune responses (green nodes), apoptosis (blue), angiogenesis (yellow), proliferation (red), adhesion (gold), proteolysis (light red) and other processes (light blue). The networks were presented by cytoscape.

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques:

A, a network of hypopharyngeal cancer. B, a network of oral cancer. Every node represents a common target gene of NF-κB, p53, mir21 or mir34ac, and was annotated to inflammatory and immune responses (green nodes), apoptosis (blue), angiogenesis (yellow), proliferation (red), adhesion (gold), proteolysis (light red) and other processes (light blue). The networks were presented by cytoscape.

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: A, a network of hypopharyngeal cancer. B, a network of oral cancer. Every node represents a common target gene of NF-κB, p53, mir21 or mir34ac, and was annotated to inflammatory and immune responses (green nodes), apoptosis (blue), angiogenesis (yellow), proliferation (red), adhesion (gold), proteolysis (light red) and other processes (light blue). The networks were presented by cytoscape.

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques:

UM-SCC 22B (A, the mt p53) and 1 (B, the wt p53-deficient) cells were transfected with siRNA to RelA or TP53 for 48 h or 72 h. Total RNA was isolated, and genes selected from TF-miRNA networks in the were analyzed by real time qRT-PCR. The data were calculated as the mean plus standard deviation from triplicates with normalization by 18S ribosome RNA, and presented as the comparison with the cultured cells transfected with the control siRNA oligos. The open bar represents the cells transfected with control siRNA, and the values have been normalized to 1. The fold changes of the target genes were presented when compared with those transfected with control siRNA. The black bar represents cells transfected with siRNA targeting TP53, while the blue bar represents cells transfected with siRNA targeting NF-κB subunit, RelA/p65. * refers to statistical significance (t test, P <0.05).

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: UM-SCC 22B (A, the mt p53) and 1 (B, the wt p53-deficient) cells were transfected with siRNA to RelA or TP53 for 48 h or 72 h. Total RNA was isolated, and genes selected from TF-miRNA networks in the were analyzed by real time qRT-PCR. The data were calculated as the mean plus standard deviation from triplicates with normalization by 18S ribosome RNA, and presented as the comparison with the cultured cells transfected with the control siRNA oligos. The open bar represents the cells transfected with control siRNA, and the values have been normalized to 1. The fold changes of the target genes were presented when compared with those transfected with control siRNA. The black bar represents cells transfected with siRNA targeting TP53, while the blue bar represents cells transfected with siRNA targeting NF-κB subunit, RelA/p65. * refers to statistical significance (t test, P <0.05).

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques: Transfection, Isolation, Quantitative RT-PCR, Standard Deviation, Comparison, Cell Culture, Control

Nuclear extract from UM-SCC 1 cells were isolated, and the binding activity was examined on the biotin labeled oligos containing promoter sequences with p53 binding sites. The binding activity of nuclear extract of MCF-7 cells with a biotinylated oligonucleotide containing a known p53 binding site in the CDKN1A gene promoter (“MCF-7 p21 oligo”) was served as a positive control. Specificity of binding in the UM-SCC cells was also tested using cold competition (unbiotinylated) oligonucleotides which contained the wild-type p53 consensus binding sequence (“wt competition”) or mutant DNA sequence of p53 binding site (“mt competition”).The mean and standard deviation of each binding was calculated from triplicate, and the presented data are from one representative of repeated experiments. *refers to statistical significance when p53 binding activity was competed by the mt oligo, to confirm the binding specificity (t test, P <0.05).

Journal: PLoS ONE

Article Title: Unraveling Regulatory Programs for NF-kappaB, p53 and MicroRNAs in Head and Neck Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0073656

Figure Lengend Snippet: Nuclear extract from UM-SCC 1 cells were isolated, and the binding activity was examined on the biotin labeled oligos containing promoter sequences with p53 binding sites. The binding activity of nuclear extract of MCF-7 cells with a biotinylated oligonucleotide containing a known p53 binding site in the CDKN1A gene promoter (“MCF-7 p21 oligo”) was served as a positive control. Specificity of binding in the UM-SCC cells was also tested using cold competition (unbiotinylated) oligonucleotides which contained the wild-type p53 consensus binding sequence (“wt competition”) or mutant DNA sequence of p53 binding site (“mt competition”).The mean and standard deviation of each binding was calculated from triplicate, and the presented data are from one representative of repeated experiments. *refers to statistical significance when p53 binding activity was competed by the mt oligo, to confirm the binding specificity (t test, P <0.05).

Article Snippet: p53 DNA binding activity was quantitatively assessed using a modified version of the TransAM p53 ELISA kit from Active Motif (Carlsbad, CA).

Techniques: Isolation, Binding Assay, Activity Assay, Labeling, Positive Control, Sequencing, Mutagenesis, Standard Deviation